Anti-Flag
antibody
Catalog: RGF2030
Product Information：
Description: Mouse monoclonal antibody
Synonyms: DYKDDDDK tag
Immunogen: Synthetic peptide of fragment (DYKDDDDK).
Form:
Liquid，20μl，1mg/ml
Purification: Protein A purified
Stability
& Storage: Use a manual defrost freezer and avoid repeated freeze-thaw
cycles. 12 months from date of receipt, -20 to -70 ℃as supplied. 1
month, 2 to 8 ℃ under sterile conditions
after reconstitution.
Application Information：
Recommended
Dilution: Western Blot (1:1000-1:2000)
Note:
Optimal dilutions/concentrations should be determined by the end user.
Confirmed
Reactivity: Anti-DYKDDDDK Tag recognizes
DYKDDDDK-tagged fusion proteins.
Application Example
Lane
1: 30 ng DYKDDDDK-tagged fusion protein.
Lane
2: 50 ng DYKDDDDK-tagged fusion protein.
Lane
3: 75 ng DYKDDDDK-tagged fusion protein.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer:
blotting to NC (nitrocellulose) membrane for 1 h.
Blocking:
5% skim milk at RT or 4℃ for 1 h.
Primary
antibody: 1:2000 dilution overnight at 4℃.
Secondary
antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP)
Detection:
using chemiluminescence substrate and image were captured with CCD camera.