Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.
貨號：C0204.0050
規(guī)格：50升
價(jià)格：600元
產(chǎn)地：Duchefa
N6
Medium(CHU Medium)
Total
concentration Micro and Macro elements including vitamins: 3956.53 mg/l
|
Macro Elements
|
mg/l
|
mM
|
|
CaCl2
|
125.33
|
1.13
|
|
KH2PO4
|
400.00
|
2.94
|
|
KNO3
|
2830.00
|
27.99
|
|
MgSO4
|
90.27
|
0.75
|
|
(NH4)2SO4
|
463.00
|
3.50
|
|
Micro Elements
|
mg/l
|
μM
|
|
FeNaEDTA
|
36.70
|
100.00
|
|
H3BO3
|
1.60
|
25.88
|
|
KI
|
0.80
|
4.81
|
|
MnSO4.H2O
|
3.33
|
19.70
|
|
ZnSO4.7H2O
|
1.50
|
5.22
|
|
Vitamins
|
mg/l
|
μM
|
|
Glycine
|
2.00
|
26.64
|
|
Thiamine HCl
|
1.00
|
2.96
|
|
Pyridoxine HCl
|
0.50
|
2.43
|
|
Nicotinic acid
|
0.50
|
4.06
|
|
|
|
|
Chu C.C, The N6 medium and its
application to anther culture of -cereal crops, Proc.
Symp. Plant Tissue Cult., Peking, 43 (1978).
Chu C.C. et al., Establishment of an
efficient medium for anther culture of rice through comparative experiments on
the nitrogen -sources. Scienta Sinic.,
18, 659 (1975).